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  • Title: Glucose transport carrier of human erythrocytes. Radiation target size measurement based on flux inactivation.
    Author: Cuppoletti J, Jung CY, Green FA.
    Journal: J Biol Chem; 1981 Feb 10; 256(3):1305-6. PubMed ID: 7192711.
    Abstract:
    Intact human erythrocytes frozen in the presence of cryoprotective reagents and irradiated with an electron beam retained their diffusion barrier to L-glucose. The carrier-mediated flux of D-glucose, on the other hand, was inactivated as a simple exponential function of the radiation dose. Classical target size analysis of this data yielded a molecular size of 185,000 daltons for the carrier. This represents the first measurement of the functional size of a transport protein based directly on flux inactivation.
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