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  • Title: Determination of SHBG-bound sex hormones by selective ammonium sulphate precipitation.
    Author: Ratajczak T, Monaco EM, Hähnel R.
    Journal: Clin Chim Acta; 1981 Mar 05; 110(2-3):327-34. PubMed ID: 7194750.
    Abstract:
    This paper describes a direct method for determining sex hormone binding globulin (SHBG)-bound sex hormones in human plasma after separation of SHBG-bound and unbound hormone fractions by selective precipitation with ammonium sulphate. In normal women variations in SHBG-bound and -free hormone generally paralleled fluctuations in total hormone. Changes in SHBG-free estradiol did not have any marked effect on plasma SHBG and sHBG-free testosterone. Our results suggest a buffer role for SHBG through which the biological response to sudden changes in sex hormone concentration is moderated.
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