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  • Title: [Detection of lymphocytotoxic HLA-A, B, C antibodies in the blood of married female blood donors. I. Serological study].
    Author: Diotallevi P, Guerra M.
    Journal: Boll Soc Ital Biol Sper; 1981 Feb 28; 57(4):428-34. PubMed ID: 7195266.
    Abstract:
    Bleedings from 1.843 blood donors married women have been checked, according to NIH standard technique, against panels of 17-20 samples of lymphocytes from random healthy HLA-A, -B, -C typed individuals of the Turin population. Lymphocytotoxic activity has been found in 209 sera, but only 90 of them showed strong reaction(s) (at least 60-80% of killed lymphocytes) and 20 of these were HLA typing (or easily transformable in HLA typing) sera. Very few sera killed only 20-50% of lymphocytes in some samples and this cytotoxic activity remained even if dilutions as far as 1:16 and, sometimes, 1:32 were made. Bleedings from blood donors married women give a small yield (1%) of ;typing sera, but these sera are easily available and, practically, without problems of quantity.
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