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  • Title: Direct detection of two cross-reactive antigens between porcine and rabbit zonae pellucidae by radioimmunoassay and immunoelectrophoresis.
    Author: Wood DM, Dunbar BS.
    Journal: J Exp Zool; 1981 Sep; 217(3):423-33. PubMed ID: 7200123.
    Abstract:
    Zonae pellucidae were isolated from oocytes obtained from rabbit and porcine ovaries. Antisera were prepared in rabbits against heat-solubilized porcine zonae and in guinea pigs against intact and heat-solubilized rabbit zonae and against purified rabbit zona proteins isolated from two-dimensional electrophoresis gels. These antisera were used in radioimmunoassay studies to show cross-reactivity between porcine and rabbit zona antigens. Immunoelectrophoresis studies also demonstrated the immunochemical cross-reactivity between two distinct antigens of rabbit and porcine zonae. These two rabbit zona antigens were demonstrated to be immunochemically identical to porcine zona antigens by crossed-line immunoelectrophoresis.
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