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  • Title: The subunit structure of bovine heart mitochondrial transhydrogenase.
    Author: Anderson WM, Fisher RR.
    Journal: Biochim Biophys Acta; 1981 Mar 12; 635(1):194-9. PubMed ID: 7213674.
    Abstract:
    Reaction of purified bovine heart transhydrogenase with bifunctional cross-linking reagents dimethyl adipimidate, dimethyl pimelimidate, dimethyl suberimidate, and dithiobis(succinimidyl propionate) results in the appearance of a dimer band on sodium dodecyl sulfate polyacrylamide gels with no higher oligomers formed. Treatment of the enzyme with 6 M urea led to inactivation and prevented cross-linking by dimethyl suberimidate. Transhydrogenase reconstituted into phosphatidylcholine proteoliposomes also yielded a dimer band on cross-linking. These data indicate that soluble and functionally reconstituted transhydrogenase possesses a dimeric structure.
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