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  • Title: A simple chromatographic technique for the estimation of urinary levels of normetadrenaline and metadrenaline.
    Author: Hanson P, Smith JR, Robinson R, Stott AW.
    Journal: Clin Biochem; 1980 Dec; 13(6):262-5. PubMed ID: 7214693.
    Abstract:
    A simple, but effective, procedure is described for the concurrent estimation of normetadrenaline (NMA) and metadrenaline (MA) in urine. The 3-methoxycatecholamines are adsorbed by a cation exchange resin, desorbed, separated by paper chromatography, and finally estimated spectrophotometrically after oxidation to vanillin. The mean (+/- 2 standards deviations) urinary outputs of NMA and MA from 123 normals (80 males, 43 females) in 24 hours was 2.15 +/- 1.04 and 1.79 +/- 0.96 mu mol respectively. There was no significant difference in the 24-hour urinary outputs of NMA between the male and female groups. However, the output of MA from the males was significantly higher than that from the females.
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