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  • Title: Preparation and assessment of frozen-hydrated sections of mammalian tissue for electron microscopy and X-ray microprobe analysis.
    Author: Ross A, Sumner AT, Ross AR.
    Journal: J Microsc; 1981 Mar; 121(Pt 3):261-72. PubMed ID: 7218347.
    Abstract:
    A system is described for preparing and examining frozen-hydrated sections of mammalian tissue, using commercially available equipment. An essential part of the system is a liquid nitrogen cooled vacuum transfer system. Minor modifications were required to interface the various manufacturers' equipment, and also to facilitate handling of specimens in the cryoultramicrotome. A variety of criteria indicates that our sections are truly frozen-hydrated, but negligible image contrast in the sections is a serious problem both for morphological work and X-ray microanalysis.
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