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  • Title: [Complex isolation of intra- and extracellular RNases from Aspergillus clavatus].
    Author: Beletskaia OP, Ivanova GS.
    Journal: Prikl Biokhim Mikrobiol; 1980; 16(4):554-60. PubMed ID: 7220511.
    Abstract:
    A scheme for isolating guanyl-specific and -nonspecific intracellular and extracellular RNases from the fungus Aspergillus clavatus cultivated in a fermenter has been developed. The scheme involves chromatography on DEAE and CM-celluloses, QAE Sephadex A-50 gel filtration on Sephadex G-75 and ultragel A-44, and crystallization. Using the scheme, four electrophoretically homogeneous enzymes have been purified, two of them being crystalline guanyl-specific RNases and two nonspecific RNases.
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