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  • Title: [Problems relating to low temperature preservation of ram semen. 1. Effects of various cryoprotective agents on sperm].
    Author: Neubert L, Menger H.
    Journal: Arch Exp Veterinarmed; 1981; 35(1):51-6. PubMed ID: 7224792.
    Abstract:
    Studies into the action of several cryoprotective agents in sodium-citrate-yolk diluent on ram semen provided preliminary information regarding both concentration applicable in deep-freeze preservation and toxicity of the agents involved. Included in the tests were glycerin, dimethylsulphoxide, ethylene glycol, propylene glycol, polyethylene glycol, polyvinylpyrrolidome, and dimethylacetamide. The effect of adding cryoprotective-containing diluent at 5 degrees C was more favourable, in its impact on ram sperma, than that observed, when diluent was added at 30 degrees C.
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