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  • Title: Determination of monoacetyldiamines and -polyamines in urine by high-performance liquid chromatography.
    Author: Abdel-Monem MM, Merdink JL.
    Journal: J Chromatogr; 1981 Mar 13; 222(3):363-70. PubMed ID: 7228946.
    Abstract:
    A procedure is described for the determination of monoacetylputrescine, N1-acetyl-spermidine and N8-acetylspermidine in human urine. The procedure is based on the high-performance liquid chromatographic separation of the 5-dimethylaminonaphthalene-1-sulfonyl (dansyl) derivatives of these amines using two different chromatographic modes. Monoacetyl-1,6-diaminohexane was used as an internal standard. The amines were extracted from urine using a silica gel cartridge. The dansyl monoacetylpolyamines were separated from the mixture of dansyl derivatives of urinary amines on a bonded-phase CN column using a programmed solvent gradient elution. The dansyl acetylpolyamines were rechromatographed on a silica gel column. This chromatographic procedure was used for the determination of the concentration of N1-acetylspermidine, N8-acetylspermidine and monoacetylputrescine in the urine of healthy volunteers and cancer patients.
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