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  • Title: Expression of cell-surface latent allotypes: latent specificities are synthesized only by cells that display the nominal allotype.
    Author: Wolf B, Currie EP, Yarmush M.
    Journal: J Immunol; 1981 Jun; 126(6):2490-3. PubMed ID: 7229384.
    Abstract:
    The association of latent and nominal allotypes at the cell surface of rabbit peripheral blood lymphocytes (PBL) was studied in animals from 2 closed group colonies. The specificity of the anti-allotype antibodies used in the mixed antiglobulin rosette test was confirmed by radioimmune assay and by testing PBL from latent allotype-free homozygotes. PBL from rabbits exhibiting latent group a and b allotypes were treated with pronase to bring the rosetting population down to less than 1 to 2%; latent and nominal cell-surface allotypes were then regenerated overnight in serum-free culture. Latent and nominal allotypes were detected in the same cell in 16 to 57% of the total allotype-bearing population. The remaining cells displayed only nominal allotype. This was observed in rabbits bearing latent a1, a2, a3, b4, b5, and b6. Rosette-inhibition data indicated that some of the group a latent and nominal allotypes were contiguously associated at the cell surface but that the group b latent and nominal markers were spaced further apart. In addition, in rabbits exhibiting 2 latent group b allotypes, both single and double latent cell surface expression was seen. Rabbits from the Philadelphia and N.I.H. internally derived colonies gave similar results.
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