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  • Title: [Mechanism of ATP-dependent DNAse purified from sea urchin Strongylocentrotus intermedius embryos].
    Author: Gafurob IuM, Sibirtsev IuT, Skoblov IuS, Rasskazov VA.
    Journal: Mol Biol (Mosk); 1980; 14(1):76-85. PubMed ID: 7231406.
    Abstract:
    Supercoiled DNAs of the SV40 virus and phi X174 phage have been studied under the effect of ATP-dependent DNase purified from sea urchin embryos. The studies showed a relaxing activity of the enzyme in relation to the supercoiled DNA. The supercoiled DNA treated with the enzyme in the absence of ATP was ultracentrifuged in a linear (5-20%) alkali sucrose gradient and the DNA preparations obtained by Davis formamide technique were examined by electron microscopy to demonstrate accumulation of double stranded DNA (RFIII). After addition of ATP to the incubation mixture only relaxed (RFII) and supercoiled (RFI) molecules of circular DNAs were observed among the products of enzyme hydrolysis of supercoiled DNAs.
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