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  • Title: The nature of the RNA products synthesized in vitro with a cell-free extract from TMV-infected tobacco leaves.
    Author: Rongxiang F, Xiaofeng W, Bingyi Q, Faxing C, Ming B.
    Journal: Sci Sin; 1980 Mar; 23(3):357-70. PubMed ID: 7233151.
    Abstract:
    A cell-free extract containing TMV-RNA replicase was prepared from TMV-infected tobacco leaves. It could synthesize double-stranded RNAs in the presence of four nucleoside triphosphates (among them, UTP was tritium-labelled), magnesium ion and actinomycin D. It was confirmed by polyacrylamide-agarose gel electrophoresis, RNase treatment, thermal denaturation and self annealing that 3H-ds RNAs, obtained from phenol-SDS extraction and Serva cellulose column chromatography, consisted of replicative form (RF) and replicative intermediate (RI) of TMV-RNA, with molecular weights of 40 X 10(6) and 5.0 X 10(6), respectively. Molecular hybridization competition experiment showed that 60-70% of the nascent RNAs in the 3H-ds RNA were plus strand of tMV-RNA.
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