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  • Title: Highly purified mitochondria from rat brain prepared by phase partition.
    Author: López-Pérez MJ, París G, Larsson C.
    Journal: Biochim Biophys Acta; 1981 Apr 13; 635(2):359-68. PubMed ID: 7236669.
    Abstract:
    Mitochondria and synaptosomes from adult rat forebrain can easily be separated by counter-current distribution in an aqueous two phase system composed of Dextran T500 and poly(ethylene glycol) 4000. Both particles may also be separated by a batch procedure in which the same phase system is used. Electron micrographs and enzymatic activities show a high purity of the mitochondria obtained from the dextran-rich lower phase. Electron micrographs and enzymatic activities also show that intact synaptosomes can be obtained from the poly(ethylene glycol)-rich upper phase. The mitochondria purified by this method show good ADP/O ratios, respiratory control ratios, and state 3 rates. Synaptosomes showed a state 2-state 3 transition with no recuperation to state 4.
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