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  • Title: Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing.
    Author: Axelrod VD, Vartikyan RM, Aivazashvili VA, Beabealashvili RS.
    Journal: Nucleic Acids Res; 1978 Oct; 5(10):3549-63. PubMed ID: 724495.
    Abstract:
    Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E. coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D delta III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.
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