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  • Title: The effect of glucosone on the proliferation and energy metabolism of in vitro grown Ehrlich ascites tumor cells.
    Author: Reiffen KA, Löffler M, Schneider F.
    Journal: Z Naturforsch C Biosci; 1981; 36(3-4):255-61. PubMed ID: 7245839.
    Abstract:
    1) Proliferation and energy metabolism of in vitro growth Ehrlich ascites tumor (EAT) cells in the presence of glucosone, (D-arabino-3.4.5.6-tetrahydroxy-2-oxo-hexanal) a competitive inhibitor of hexokinase, were studied. 2) Proliferation of the cells was completely inhibited by 2 mM glucosone without severely affecting viability (dye exclusion test). No phase specific arrest of cell growth was observed. 3) Incorporation of [14C]thymidine into an acid insoluble fraction of the cells decreases to 5% of the control within 8-10 h. Incorporation of [14C]leucine begins to slow down immediately after treatment with glucosone. 4) The inhibitor (2 mM) reduces the lactate production of the cells by 60%, respiration by about 20%; the ATP/ADP ratio slows down from 4.75 to 3.5. 5) The total inhibition of cell proliferation by 2 mM glucosone cannot be explained exclusively by inhibition of hexokinase activity and impairment of energy metabolism. Because of a lack of specificity, glucosone is not a suitable inhibitor for studies on the relationship between hexokinase activity and cell proliferation of tumor cells.
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