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  • Title: [Effect of medium components on the metabolism of Brevibacterium ammoniagenes-producer of 5'-inosinic acid].
    Author: Lukin NS, Kazarinova LA, Erokhina LI.
    Journal: Prikl Biokhim Mikrobiol; 1978; 14(4):565-72. PubMed ID: 724664.
    Abstract:
    The effect of different carbon and nitrogen sources on the biosynthesis of 5'-inosinic acid (5'-IMP) by the culture Brevibacterium ammoniagenes 225-5 was studied. With respect to the yield of 5'-IMP, glucose was found to be the best carbon source and urea--the best nitrogen source. The proper ratio of concentrations of carbon and nitrogen sources in the nutrient medium was very important for a high 5'-IMP accumulation. The study of the effect of phosphate and magnesium concentrations on 5'-IMP biosynthesis demonstrated that their high concentrations were needed for intensive 5'-IMP salvage-synthesis from hypoxanthine and that they did not influence the de novo synthesis of hypoxanthine.
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