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  • Title: Specificity of non-histone proteins from hamster Kirkman-Robbins hepatoma.
    Author: Gaczyński M, Kiliańska Z, Graczyk G, Kłyszejko-Stefanowicz L.
    Journal: Neoplasma; 1980; 27(6):653-9. PubMed ID: 7254423.
    Abstract:
    Nuclear phosphoproteins from Syrian hamster liver and Kirkman-Robbins hepatoma were obtained by phenol method of TENG et al. and their chemical composition was investigated. Amino acid composition of phosphoproteins from both tissues resembled each other to a great extent. There was almost twice more phosphorus in the preparations from hepatoma than in the ones from liver. Using two electrophoretic techniques: SDS-polyacrylamide gel electrophoresis and isoelectric focusing some differences between the examined proteins of neoplastic and normal tissue were found. Three additional fractions (molecular weights 29 000, 89 000 and 93 000, respectively) could be observed in case of hepatoma proteins in comparison with the ones from liver. The phosphoproteins of Kirkman-Robbins hepatoma revealed three additional bands with isoelectric points 6.6, and 7.1.
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