These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Affinity chromatography of some pyridoxal phosphate-requiring enzymes on Cibacron Blue F3GA-agarose.
    Author: Meadows GG, Cantwell GS.
    Journal: Res Commun Chem Pathol Pharmacol; 1980 Dec; 30(3):535-45. PubMed ID: 7255863.
    Abstract:
    Tyrosine phenol-lyase from Erwinia herbicola was purified from a cell-free extract in a single step on Cibacron Blue F3GA-agarose. The protein was purified as the apoenzyme and was unstable after affinity chromatography. Alanine aminotransferase and aspartate aminotransferase from porcine heart also bound to Cibacron Blue F3GA-agarose. These enzymes were partially purified as holoenzymes from a crude porcine heart extract by elution with NADH and KCl. Alanine aminotransferase was purified 19 fold by this procedure.
    [Abstract] [Full Text] [Related] [New Search]