These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Measurement of triiodothyronine in urine.
    Author: Yoshida K, Sakurada T, Kaise N, Yamamoto M, Kaise K, Saito S, Yoshinaga K.
    Journal: Tohoku J Exp Med; 1980 Dec; 132(4):389-95. PubMed ID: 7256725.
    Abstract:
    A radioimmunoassay procedure to measure triiodothyronine (T3) in unextracted urine is described. One hundred microliter of T3 standards or samples were incubated with 100 microliter of T3 antiserum (1:40,000), 100 microliter of tracer 125I-T3 and 700 microliter of 0.05 M borate buffer, pH 8.6. Separation of free from bound antigen was achieved by dextran coated charcoal. The cross-reaction of L-T3 with L-T4 in this assay system was less than 0.2%. Dilution of high T3 urine was parallel to the standard curve. Recovery was 101 +/- 8%. Coefficients of variation were 3-8% within each assay and 13% between assays. Euthyroid subjects excreted 0.81 +/- 0.39 microgram (mean +/- S.D.) in 24 hr. Similar values were obtained in pregnant women, euthyroid patients with chronic thyroiditis, and a patient with thyroxine-binding globulin deficiency. Excretion of urinary T3 was high (7.48 +/- 3.32 microgram/24 h) in patients with hyperthyroidism, and low (0.14 +/- 0.15 microgram/24 hr) in patients with hypothyroidism. Urinary T3 excretion was almost undetectable in terminal renal failure. Positive correlations were found between urinary T3 and serum total T3 (r = 0.89) and especially serum free T3 (r - 0.97). A positive correlation was also found between urinary T3 and creatinine clearance (r = 0.63). Mean urinary clearance of T3(CT3=Urinary T3 excretion/serum free T3 was 166 ml/min and was significantly higher than their mean creatinine clearance (110 ml/min). The measurement of T3 in urine is reliable and easy to perform, and may allow a new approach to the understanding of thyroid hormone metabolism.
    [Abstract] [Full Text] [Related] [New Search]