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  • Title: Blastogenic response of swine lymphocytes in whole blood, purified blood, spleen cell, and lymph node cell cultures with concanavalin A and phytohemagglutinin.
    Author: Hussain A, Tripathy RN, Mohanty SB, Rockemann DD.
    Journal: Am J Vet Res; 1981 May; 42(5):873-5. PubMed ID: 7258810.
    Abstract:
    Microcultures of swine whole blood, purified blood lymphocytes (PBL), spleen cells, and lymph node (LN) cells were tested for stimulation by concanavalin A (conA) and phytohemagglutinin (PHA). A cell concentration of 2 x 10(6)/ml and a 1:20 dilution of whole blood were needed for optimal stimulation of conA and PHA. Optimal stimulation of whole blood lymphocytes (WBL) and LN cells was obtained by 20 micrograms of conA/ml, but 10 micrograms/ml of this mitogen was adequate for spleen cells. The PBL and LN cells were optimally stimulated by 20 microliters of PHA/ml, but 80 microliters and 40 microliters/ml of this mitogen were required for stimulating the whole blood and spleen cells, respectively. Four days' incubation was needed for maximal blastogenesis of WBL with conA. This could be achieved after 3 days' incubation with PBL and spleen and LN cells. Whole blood and spleen cells were stimulated by PHA after 3 days' incubation, but PBL and LN cells required 4 days' incubation. Reproducibility, sensitivity, and comparison of various cultures were discussed.
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