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  • Title: Permeability to ions of bovine retinal disk membrane vesicles in the bleached state.
    Author: Amis EJ, Wendt DJ, Erickson ED, Yu H.
    Journal: Biochim Biophys Acta; 1981 Jun 22; 644(2):201-10. PubMed ID: 7260074.
    Abstract:
    The permeability of the bleached disk membrane of retinal rod outer segments to univalent and divalent ions is studied by light scattering. The membranes are isolated from frozen dark-adapted bovine retinae, swollen into spherical vesicles in a hypotonic medium and bleached in dilute suspension and their size is determined by elastic and quasi-elastic light scatterings. Various electrolytes are then added to the suspending medium in order to examine their osmotic activity relative to the vesicles deformation characteristics. By following the deformation behavior of the membrane vesicles by elastic light scattering in terms of the oblate ellipsoidal shell model, the osmotic activity of a given electrolyte is qualitatively deduced and thereby the permeability of the membrane to the electrolyte is ranked in reference to a chosen standard, i.e., sucrose. By this method, we show that the permeabilities to Na+, K+, Mg2+ and Ca2+ are all alike, and those to halides (F-, Cl-, Br-, I-), nitrate and phosphates (HPO4(2-)/H2PO4-) are similar. Acetate, however, is about 3-times more permeative, while sulfate is less permeative than the other anions by about the same factor. The viability of our method is checked with use of an ionophore, lasolocid (X-537A), by establishing partial recovery from the osmotic deformation through the suppression of the cation osmotic effect. Ion-induced aggregation and pH-dependent size and shape changes are both found to be insignificant.
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