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  • Title: Purification of an alkaline phosphatase-lipoprotein-X complex.
    Author: Weijers RN, Kruijswijk H, Baak JM.
    Journal: Clin Chem; 1980 Apr; 26(5):604-8. PubMed ID: 7261305.
    Abstract:
    An alkaline phosphatase isoenzyme was observed in an abnormal position in the agar-agarose gel pattern of sera from several patients suffering from intrahepatic and extrahepatic cholestasis. We purified this isoenzyme, by gel filtration and affinity chromatography, from the serum of a patient suffering from extrahepatic cholestasis. Analysis demonstrated an alkaline phosphatase-lipoprotein-X complex with a relative molecular mass of at least 669,000. We discuss the interpretation of alkaline phosphatase isoenzyme patterns produced by different techniques.
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