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  • Title: Rapid OTAN method for localizing unsaturated lipids in lung tissue sections.
    Author: Negi DS, Stephens RJ.
    Journal: Stain Technol; 1981 May; 56(3):177-80. PubMed ID: 7268814.
    Abstract:
    The OTAN treatment, which is the only histochemical method available at present for the simultaneous localization of hydrophobic and hydrophilic unsaturated lipids in tissue sections, requires unduly long exposure to OsO4 and use of free-floating sections, which makes handling the sections difficult and often results in their loss or damage. Simple modifications using OsO4 treatment at 37 C and slide-mounted sections eliminate the practical drawbacks of the existing method and provide as good or better localization in less than one-eight of the time. The modified method is applicable to fixed as well as fresh frozen tissues.
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