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  • Title: [Cell wall polysaccharide structure in the food protein yeast, Candida spec. H. II. Characterization of phosphate binding to the mannan-protein-phosphate complex and identification of phosphodiester-bound mono- oligosaccharides].
    Author: Grimmecke HD, Reuter G.
    Journal: Z Allg Mikrobiol; 1981; 21(2):109-16. PubMed ID: 7269644.
    Abstract:
    The proteophosphomannan (PPM) obtained by extraction with citrate buffer, purification by the cetavlon method and gel filtration contains a high proportion of diesterified phosphate groups between the C-6 of a side chain mannose and another glycosidically bound carbohydrate. Mild acid hydrolysis cleaved the phosphate diester linkages to yield mono- and oligosaccharide fractions. Chemical analysis and 1H-NMR studies demonstrated the heterogeneity of the oligosaccharide fractions containing alpha(1,2)-, alpha(1,3)- and alpha(1,6)-linked manno-oligosaccharides. All repeated PPM preparations contained mono-, tri- and heptasaccharides as the phosphate bound main compounds. Galactose, arabinose, fucose, and rhamnose of the monosaccharide fraction and tetra-, penta- and hexasaccharides were not found in all preparations.
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