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  • Title: [Comparison between quantitative determinations of blood protein fractions of clinically healthy cattle using cellulose acetate film and micro-agar gel electrophoresis].
    Author: Blauärmel H.
    Journal: Arch Exp Veterinarmed; 1978; 32(4):525-30. PubMed ID: 727867.
    Abstract:
    Cellulose acetate sheet and agar electrophoresis was used to test the plasma of 106 clinically intact Frisians for total protein and "classical fractions". The average total protein recorded from plasma samples was 6.9 g/100 ml. Plasma albumin concentrations measured by cellulose acetate sheet electrophoresis were 11.39 per cent in excess of those obtained by agar electrophoresis, all at the expense of the alpha1, alpha2, and beta globulins. The results obtained from the two variants of electrophoresis differed from one another by 99 per cent of statistical security for albumin, as well as for alpha1, alpha2, and beta globulins. No such significant difference, however, was established for gamma globulins. It is thought that the low amount of albumin in response to agar electrophoresis was attributable to the fact that part of the albumin fraction had been absorbed to the agar. In pherogram evaluation it would then be calculated as being identical with the subsequent and more slowly travelling fractions of the alpha1, alpha2, and beta globulins. Immuno-electrophoretic experiments have shown the impossibility of real separation of alpha1 globulins from albumin on agar with antiserum absent. The amount of alpha1 globulin, therefore, was relatively high in agar electrophoresis. The electrophoretic approach, consequently, must be considered in any interpretation of proteinograms to avoid error.
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