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  • Title: [Ultrastructure of the mitotic apparatus in metaphase cells exposed to 2-mercaptoethanol in embryonic swine kidney tissue culture].
    Author: Bystrevskaia VB, Onishchenko GE, Chentsov IuS.
    Journal: Tsitologiia; 1981 Jul; 23(7):745-52. PubMed ID: 7281226.
    Abstract:
    The ultrastructure of the mitotic apparatus in metaphasic cells of the porcine embryo kidney culture was studied in the course of the 20 hour treatment with 2-mercaptoethanol (0.001M). In these conditions metaphasic cells were recognized according to the following signs: chromosome disposition; the degree of spindle microtubule destruction; the structure of spindle poles, and the organization of kinetochore regions. On this basis, five types of metaphasic cells have been distinguished. It is suggested that modifications of the mitotic apparatus in these metaphasic types may reflect the reorganization of all its parts (metaphasic plate, mitotic spindle, spindle poles) that occur in the process of the transformation of mitotic cells, from the normal metaphase stage to the stage of C-metaphase under the influence of 2-mercaptoethanol treatment.
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