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Title: [Phosphorylation reactions of lysyl-tRNA-synthetase from rat liver]. Author: Kucherenko NE, Vinogradova RP, Litvinenko AR, Mirutenko NV, Verkhogliad IN. Journal: Ukr Biokhim Zh (1978); 1981; 53(5):49-54. PubMed ID: 7292621. Abstract: The phosphorylation reaction of lysyl-tRNA-synthetase from the rat liver tissue was studied in the in vivo experiments. The activity of 3':5'-AMP-dependent protein kinase from the myocardium in the phosphorylation reaction is 3.4 times lower than that of the enzyme from the liver. Optimum conditions of the lysyl-tRNA-synthetase phosphorylation reaction were determined. The Vmax and Km values for the reaction were 2.7 pmol of 32P/mg per 1 minute and 0.4-10(-4), respectively. The lysyl-tRNA-synthetase phosphorylation increases the tRNA aminoacylation.[Abstract] [Full Text] [Related] [New Search]