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  • Title: Influence of pH and ionic strength on the lysis of Micrococcus luteus cells by hen lysozyme at low (20 degree C) and high (physiological, 40 degree C) temperature.
    Author: Saint-Blancard J, Maurel JP, Constant JF, Berthou J, Jolles P.
    Journal: Biosci Rep; 1981 Feb; 1(2):119-23. PubMed ID: 7295888.
    Abstract:
    From isoactivity curves (showing activity as a function of Ph and ionic strength) it was found that in the pH domain 6.7-8.6 frequently used in experiments involving hen lysozyme, the pH optimum of lysis of Micrococcus luteus cells at low ionic strength (0.02-0.05) by the high-temperature form (40 degree C, physiological temperature) was one to two pH units lower than that by the low-temperature form (20 degree C).
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