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  • Title: High pressure liquid chromatographic determination of theobromine and caffeine in cocoa and chocolate products.
    Author: Kreiser WR, Martin RA.
    Journal: J Assoc Off Anal Chem; 1978 Nov; 61(6):1424-7. PubMed ID: 730647.
    Abstract:
    A method was developed for determining theobromine and caeffine in cocoa and chocolate products by high pressure liquid chromatography. After a simple hot water extraction, both theobromine and caffeine were separated by using a reverse phase C18 column and a mobile phase of methanol-water-acetic acid (20 + 79 + 1). Theobromine and caffeine were quantitated at 280 nm; average recoveries were 98.7 and 95.0%; and coefficients of variation were 2.31 and 3.91%, respectively.
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