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  • Title: Urinary free catecholamines determined by liquid chromatography--fluorometry.
    Author: Anderson GM, Young JG, Jatlow PI, Cohen DJ.
    Journal: Clin Chem; 1981 Dec; 27(12):2060-3. PubMed ID: 7307259.
    Abstract:
    Free norepinephrine, epinephrine, and dopamine are determined in urine after purification on a small ion-exchange column and concentration on alumina. The catecholamines are separated by reversed-phase ion-pair chromatography and are quantitated by measuring their native fluorescence (lambda ex 285 nm, lambda em 305 nm). The internal standard is dihydroxybenzylamine. Within-day CVs for the compounds ranged from 1.6 to 5.6%; between-day CVs were 6.6 to 9.8%.
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