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  • Title: Determination of internal insect infestation of wheat: collaborative study.
    Author: Trauba RL, Glaze L, McGary ED, Palmer WD, Quaife JT, Strozier J, Whiteman A.
    Journal: J Assoc Off Anal Chem; 1981 Nov; 64(6):1408-10. PubMed ID: 7309661.
    Abstract:
    An improved method has been developed for determining internal insect infestation of wheat kernels. The method involves acid hydrolysis of cracked wheat, wet sieving to remove the acid, transferring to a 2 L Wildman trap flask, deaeration by boiling, and treatment with Tween 80-Na4EDTA. Insects are extracted with light mineral oil. Reports from 6 collaborators showed that recovery was 95.92% for adult insect heads and 97.22% for larvae by the proposed method as compared with 87.05% and 6.12%, respectively, by the official method. The method has been adopted official first action.
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