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  • Title: Identification of Limulus polyphemus haemocyanin messenger RNA.
    Author: Wood EJ, Bonaventura J.
    Journal: Biochem J; 1981 May 15; 196(2):653-6. PubMed ID: 7317005.
    Abstract:
    Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
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