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  • Title: [Composition of a population of lysosomal structures sedimented during differential centrifugation of mouse liver homogenates].
    Author: Shkurupiĭ VA, Malygin AE, Bgatova NP, Korolenko TA.
    Journal: Biull Eksp Biol Med; 1981 Oct; 91(10):420-2. PubMed ID: 7317599.
    Abstract:
    Morphometry was used to measure radiuses and total volumes of lysosomal structures in hepatocytes and sinusoidal cells of male mouse liver. Basing on the data obtained attempts were made to describe possible structural and functional composition and cellular origin of lysosomes in granular functions isolated by differential centrifugation from liver homogenates. The greatest content of hepatocyte lysosomes was found in the group of particles with a radius less than 0.166 micrometer, where, according to the biochemical data, the relative specific activity (RSA) of acid phosphatase was higher as compared with cathepsin D. The fraction of particles with a radius more than 0.490 micrometer was mainly represented by lysosomes and heterophagolysosomes of sinusoidal cells that evidently determines higher RSA of cathepsin D in the fraction as compared with RSA of acid phosphatase.
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