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  • Title: Antigen-specific detection of soluble immune complexes in conglutinin-binding assays.
    Author: D'Amelio R, Brighouse G, Barnet M, Lambert PH.
    Journal: Clin Exp Immunol; 1981 Aug; 45(2):283-9. PubMed ID: 7318255.
    Abstract:
    A modification of the conglutinin-binding assay has been used for antigen identification in circulating immune complexes (IC). The complexed antigen was identified, in conglutinin-bound complexes, by antibodies or antibody fragments, specifically directed against the antigen. These antibody preparations were either radiolabelled or detected with 125I-protein A. This system was found efficient using in vitro-formed bovine serum albumin--anti-bovine serum albumin and tetanus toxoid--anti-tetanus toxoid soluble immune complexes at equivalence or at slight antigen or antibody excess. In addition, with 125I-IgG--anti-HBs and with 125I-F(ab')2--anti-HBs, we examined 44 sera from 41 patients with viral type B hepatitis and the presence of HBs was observed in 18 (40.9%) IC containing tested samples. Therefore, this method appears applicable to clinical investigation.
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