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  • Title: Coagulation studies f plasmas from healthy domesticated animals and persons.
    Author: Greene CE, Tsang VC, Prestwood AK, Meriwether EA.
    Journal: Am J Vet Res; 1981 Dec; 42(12):2170-7. PubMed ID: 7340587.
    Abstract:
    Optimal reaction conditions for the activated partial thromboplastin time (APTT), 1-stage prothrombin time (PT), and Russell's viper venom time were studied for pooled plasmas of horses, cattle, sheep, goats, swine, dogs, cats, and persons. Changes in CaCl2 and NaCl concentrations had significant effects on the APTT and PT reactions. The APTT was more sensitive than the PT to changes in CaCl2 concentration. The CaCl2 concentration recommended by the manufacturer for the APTT was suboptimal for some of plasmas of domesticated animals in ths study. Infusorial earth (Celite, activator) concentration provided by the manufacturer was optimal for plasmas of all species, with exception of horses and swine. Optimal incubation times for the APTT for the equine, bovine, ovine, and caprine plasmas were shorter than the 5 minutes recommended for human plasma. Optimal incubations for porcine and canine plasmas were longer than 5 minutes. The APTT and PT of all plasmas were stable for up to 3 hours' storage at 4 C in all species' plasmas tested with exception of the porcine plasma.
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