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  • Title: A direct demonstration of proton translocation coupled to transhydrogenation in reconstituted vesicles.
    Author: Earle SR, Fisher RR.
    Journal: J Biol Chem; 1980 Feb 10; 255(3):827-30. PubMed ID: 7356661.
    Abstract:
    Homogeneous bovine heart mitochondrial transhydrogenase was reconstituted into K+-loaded phosphatidylcholine liposomes. Transhydrogenase-catalyzed reduction of 3-acetylpyridine adenine dinucleotide by NADPH was stimulated severalfold when valinomycin was added to collapse the developing membrane potential. A rapid and extensive quenching of the fluorescence of the pH probe, 9-aminoacridine, under these conditions indicates that transhydrogenation is coupled to the acidification of the vesicle interior. A corresponding uptake of protons from the medium is demonstrated by electrode measurements that indicate 1 or less proton is translocated for each hydride ion equivalent transferred between the substrates.
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