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  • Title: Light scattering studies on alpha-crystallin from bovine eye lens.
    Author: Sano Y.
    Journal: J Biochem; 1980 Jan; 87(1):15-21. PubMed ID: 7358624.
    Abstract:
    Using the usual angular light scattering technique, we have measured the concentration dependence of reduced scattering intensity of fractionated bovine lens alpha-crystallin (alpha A-chain) in the presence of 30 mM calcium chloride and 0.1 M sodium chloride. The inverse intensity of scattered light at zero angle per unit concentration of alpha-crystallin has a significant upward curvature with increasing alpha-crystallin concentration. The third virial coefficient determined from this upward curvature was 1.24 x 10(-3) (mol 1/2 ml 1/2 g-1).
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