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  • Title: Integration and organization of Ti plasmid sequences in crown gall tumors.
    Author: Thomashow MF, Nutter R, Montoya AL, Gordon MP, Nester EW.
    Journal: Cell; 1980 Mar; 19(3):729-39. PubMed ID: 7363328.
    Abstract:
    The Ti plasmid sequences (T DNA) maintained in four unorganized crown gall tumor lines were defined by using restriction endonucleases and molecular hybridization techniques. Each tumor line contains a "core" T DNA which is apparently responsible for maintaining the transformed state; the core T DNA is colinear with the Ti plasmid and contains the Ti plasmid sequences referred to as the "common DNA"--sequences found in all Ti plasmids studied to date. A given Ti plasmid does not always give rise to the same T DNA complement. The data suggest that the majority if not all of the T DNA is integrated into plant DNA, that preferred regions of the Ti plasmid serve as the points of attachment to plant DNA and that the T DNA can be linked to more than one site in the plant genome.
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