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  • Title: Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.
    Author: Kolins MD, Epstein E, Civin WH, Weiner S.
    Journal: Clin Chem; 1980 Mar; 26(3):403-5. PubMed ID: 7363459.
    Abstract:
    We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.
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