These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Formation of 5 beta- and 5 alpha-products as major C19-steroids from progesterone in vitro in immature golden hamster testes.
    Author: Terada N, Sato B, Matsumoto K.
    Journal: Endocrinology; 1980 May; 106(5):1554-61. PubMed ID: 7363863.
    Abstract:
    Testicular homogenates from 8- to 55-day-old golden hamsters were incubated with [3H]progesterone, [14C]-progesterone, [3H]5 beta-pregnane-3,20-dione, [14C]progesterone plus [3H]5 beta-pregnane-3,20-dione, and [14C]progesterone plus [3H]5 alpha-pregnane-3,20-dione and NADPH. After incubation, the radioactive products were separated and identified by column and paper chromatographies with derivative formation and recrystallization to constant specific activity. The major C21-17-hydroxysteroids and C19-steroids formed from progesterone by the testes of immature golden hamsters were 5 beta- and 5 alpha-steroids, such as 3 alpha,17-dihydroxy-5 beta-pregnan-20-one, etiocholanolone, 5 beta-androstane-3 alpha,17 beta-diol, 3 alpha,17-dihydroxy-5 alpha-pregnan-20-one, androsterone, and 5 alpha-androstane-3 alpha,17 beta-diol. Incubation studies with the testes of the immature hamsters showed that the 5 alpha-C19-steroids were produced from progesterone mainly through 5 alpha-C21-steroids such as 3 alpha,17-dihydroxy-5 alpha-pregnan-20-one, while the 5 beta-C19-steroids were formed through androstenedione and testosterone. The major steroids formed by the testes of suckling and adult animals were 17-hydroxy-4-pregnen-3,20-dione, androstenedione, and testosterone, and little or no 5 beta- and 5 alpha-steroids were produced. The present results demonstrate for the first time that 5 beta-C19-steroids are major C19-steroid products of the gonad.
    [Abstract] [Full Text] [Related] [New Search]