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  • Title: Topography of rhodopsin in rod outer segment disk membranes. Photochemical labeling with N-(4-azido-2-nitrophenyl)-2-aminoethanesulfonate.
    Author: Mas MT, Wang JK, Hargrave PA.
    Journal: Biochemistry; 1980 Feb 19; 19(4):684-91. PubMed ID: 7370104.
    Abstract:
    Rod cell disk membranes have been photochemically reacted with the water-soluble, membrane-impermeable nitrene precursor N-(4-azido-2-nitrophenyl)-2-aminoethane-sulfonate [NAP-taurine, NAPT]. Rhodopsin, minor membrane proteins, and lipids all incorporate the (nitrophenyl)[35S]taurine (NPT) label. We also find that rhodopsin may be labeled in the dark using prephotolyzed NAPT. This reaction is presumably due to long-lived photoproducts of NAPT. NAPT modifies rhodopsin in the membrane in a selective manner; some cyanogen bromide peptides of NPT-rhodopsin contain appreciable NPT label and some contain essentially none. Precise specific radioactivities could not be determined for the [35S]NPT-peptides since loss of label from some of the peptides was observed during purification procedures. Rhodopsin's carboxyl-terminal cyanogen bromide peptides are well labeled when the protein is modified in disk membranes but the amino-terminal peptide is poorly labeled. When rhodopsin is labeled in reconstituted membranes in which both surfaces of rhodopsin are accessible to reagent, labeling of rhodopsin's amino-terminal peptide is enhanced. These results are consistent with a model in which rhodopsin's carboxyl-terminal region is located at the cytoplasmic (external) surface of disk membranes and its amino terminus is located at the intradiskal membrane surface.
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