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  • Title: Rapid batch fractionation of ryegrass pollen allergens.
    Author: Smart IJ, Knox RB.
    Journal: Int Arch Allergy Appl Immunol; 1980; 62(2):179-87. PubMed ID: 7372366.
    Abstract:
    A rapid batch fractionation procedure employing ion-exchange matrices has been used to fractionate ryegrass pollen antigens and allergens. After sequential elution through CM-Sephadex and DEAE-Sephadex, a group of four electrophoretically distinct allergens with a molecular weight of approximately 32,000 was fractionated. These allergens correspond to the group I allergens previously isolated by Johnson and Marsh. This material may be suitable for clinical use where a characterised group I allergen preparation is required.
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