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  • Title: Rapid determination of glycine- and taurine-conjugated bile acids in human bile by high-performance liquid chromatography.
    Author: Miyazaki S, Tanaka H, Horikawa R, Tsuchiya H, Imai K.
    Journal: J Chromatogr; 1980 Feb 08; 181(2):177-85. PubMed ID: 7372752.
    Abstract:
    With use of an anion-exchange packing, TSK Gel IEX 540 DEAE, for high-performance liquid column chromatography, glycine- and taurine-conjugated bile acids were separated in 10 min and detected with a differential refractometer. Human bile could be analyzed after a simple pretreatment. The purity of the peaks of glycine- and taurine-conjugated bile acids in human bile was confirmed by enzymatic determinations using 3 alpha-hydroxysteroid:NAD oxidoreductase. The molar ratios of the two forms of the conjugates (glycine/taurine ratios) in bile from normal subjects and from patients suffering from various hepatobiliary diseases were measured.
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