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  • Title: Mechanism of glucose transfer to steroids by rabbit liver microsomes.
    Author: Comerton AM, Layne DS, Williamson DG.
    Journal: Can J Biochem; 1978 Nov; 56(11):1082-5. PubMed ID: 737573.
    Abstract:
    The formation of the 3-glucosides of estrone, 17alpha-estradiol, or 17beta-estradiol by washed rabbit liver microsomes in the absence of UDP glucose (UDPG) was increased by the addition of crude lipid extracts of rabbit or pig liver and of dolichol or retinol. The reaction was stimulated by Mn2+, Mg2+, and Ca2+. Direct transfer of glucose to the steroid from added UDPG also occurred by a mechanism which was inhibited by metal ions. The two transferases can be separated from each other into the 105000 g pellet and supernatant respectively by treatment with detergents.
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