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  • Title: High-capacity method for purification of human liver hexosaminidase B using hydrophobic chromatography.
    Author: Hardwick J, Hechtman P.
    Journal: J Chromatogr; 1980 Apr 04; 190(2):385-91. PubMed ID: 7380955.
    Abstract:
    Hydrophobic chromatography was investigated as a purification procedure for human liver hexosaminidases. Both phenyl-Sepharose and valine-Sepharose have a high binding capacity of hexosaminidases. A degree of resolution between the A and B ioszymes is achieved with phenyl-Sepharose. Both hydrophobic supports must be used close to their capacity in order to recover the applied enzyme. Two purification procedures for human liver hexosaminidase B were employed, which resulted in recoveries of approximately 48 and 24% with final specific activities of 33,400 and 4840 nmole/min.mg, respectively.
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