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  • Title: Separation of cyanogen bromide peptides of collagen by means of high-performance liquid chromatography.
    Author: Black C, Douglas DM, Tanzer ML.
    Journal: J Chromatogr; 1980 Apr 04; 190(2):393-400. PubMed ID: 7380956.
    Abstract:
    Cyanogen bromide peptides of bovine collagen Types I, II nad III were analyzed using high-performance liquid chromatography (HPLC). Elution patterns of each collagen type were unique and reproducible. Elution patterns of the CNBr peptides of the alpha 1 and alpha 2 chains of Type I collagen were also unique and together accounted for the major components of Type I collagen. Analysis of the eluted peptides from HPLC of each collagen type by sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed specific patterns for each collagen. Thus, unique and reproducible HPLC chromatograms were obtained, providing a new analytical method that is simple, sensitive and rapid.
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