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  • Title: A gas chromatographic method for the determination of di- and polyamines in human urine.
    Author: Beninati S, Piacentini M, Cerù MP.
    Journal: Ital J Biochem; 1978; 27(3):156-67. PubMed ID: 738858.
    Abstract:
    A gas chromatographic method for the determination of di- and polyamines in human urine has been developed. The di- and polyamines have been extracted from standard solutions or from urine by a butanolic method modified to improve its sensitivity in relation to the low concentration of the amines in human normal urine. The quantitation is controlled by using two internal standards: 1,6-diaminohexane for Putrescine and Cadaverine, l-ephedrine for Spermidine and Spermine. Distilled water was the final solvent injected into the GLC column and the detector response was linear between 5 and 100 ng.
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