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  • Title: Measurement of faecal bile acid sulphates.
    Author: Podesta MR, Murphy GM, Dowling RH.
    Journal: J Chromatogr; 1980 Jun 13; 182(3-4):293-300. PubMed ID: 7391170.
    Abstract:
    A method is described for the measurement, by difference, of the sulphate fractions of faecal bile acids. A solvolysis step (for the deliberate hydrolysis of the bile acid sulphates) was added to the procedure of sample homogenisation, extraction, enzymatic hydrolysis and thin-layer chromatography. The bile acids were quantitated by gas-liquid chromatography of their methyl ester and trifluoroacetate methyl ester derivatives on 3% QF-1 columns. The total bile acid excretion in 15 control subjects was 603 +/- 71 mg/24 h (mean +/- S.E.M.). The major bile acid peaks (mg/24 h) were: lithocholic acid, without solvolysis 118 +/- 26 and including solvolysis 175 +/- 30; deoxycholic acid 60 +/- 8 and 90 +/- 18 and chenodeoxycholic acid 13 +/- 7 and 15 +/- 7. It was concluded that bile acid sulphates may form a considerable proportion of the total bile acids excreted in man.
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