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Title: Isolation and characterization of riboflavin-binding protein from pregnant-rat serum. Author: Muniyappa K, Adiga PR. Journal: Biochem J; 1980 May 01; 187(2):537-40. PubMed ID: 7396861. Abstract: A high-affinity riboflavin -binding protein was isolated and characterized for the first time from pregnant-rat sera by affinity chromatography on a lumiflavin-agarose column. The purified protein was homogeneous by the criteria of analytical polyacrylamide-gel disc electrophoresis, gel-filtration chromatography on Sephadex G-100 and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. It had a molecular weight of 90000+/-5000 and interacted with [14C]riboflavin with a 1:1 molar ratio with a dissociation constant (Kd) of 0.42 micron.[Abstract] [Full Text] [Related] [New Search]